Chemistry Reference
In-Depth Information
Controls
Five adjacent measurement points
detect each animal species.
Horse
Donkey
Pig
Sheep
Goat
Cattle
Chicken
Turkey
Controls
array design
Green channel (532 nm)
Red channel (635 nm)
= Cy3-labeled targets
= Cy5-labeled targets
Red channel (635 nm)
Green channel (532 nm)
ö
On-chip control systems allow the exact quality determination:
1.
Red
:
Orientation controls (Cy3-labeled probes; 10 measuring points)
2.
Yellow
:
a)
Dotted area: Priniting and homogeneity control of all DNA
measuring points (Cy3-labeled target: 45 measuring points).
b)
Full line area: Hybridization control (Cy3-labeled targets;
5 measuring points)
3.
Turquoise
:
PCR control (Cy5-labeled PCR products; 5 measuring points)
5.
Violet
:
Species identification probes (Cy5-labeled PCR products,
5 measuring points for each species)
Abb. 5.5
CarnoCheck
®
-Testsystem zum parallelen Nachweis von acht Tierarten auf zwölf Micro-
arrays pro Biochip. Die Detektion erfolgt in zwei Fluoreszenzkanälen -
rot:
fünf Testspots pro
Nachweissystem (Cy5);
grün:
Printkontrolle aller Spots (Cy3). (www.gbo.com/bioscience)
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