Morphological Assessment of Microcirculation - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 470

India ink: kept at room temperature.
Methyl salicylate: kept at room temperature.
Glycerol: kept at room temperature.
(c) Microangiography.
Microbarium (micropaque, baroperse, chromopaque, etc.):
powder.
Dissolves before use.
Gelatin.
3. Methods
1. Direct observation of microcirculatory vessel wall ( 4, 5 ).
Out of intraparenchymal vessels, the arteriole can be an object
for evaluation, since capillary had better be observed as its den-
sity. Evaluation should be done taking the depth from brain
surface into account since intraparenchymal microvessels are
distributed as shown in Fig. 1 .
In light microscopy, paraffi n-embedded brain is sliced from
the brain surface with the brain surface on the top. The depth
from the brain surface is grossly calculated by slice counts and
slice thickness (Fig. 2a ).
In transmission electron microscopy, a specimen of the
depth to be aimed for is taken out, embedded, and sliced,
Fig. 1. Distribution of intraparenchymal microvessels.