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In-Depth Information
Table 1
In vivo assessment of cerebral microcirculation
Surface microcirculation
Pial arterial RBC fl ow
Biomicroscopic observation
Microcinematography
High-speed microcinematography
Television biomicroscopy
Photoelectric method
Pial arterial plasma fl ow
Fluorescence angiography
Coomassie blue infusion technique
Infrared absorption angiography
Pial arterial blood fl ow
Laser-Doppler fl owmetry
Pial arterial pressure
Micropipet method
Parenchymal microcirculation
Regional hemoglobin content and mean transit time: photoelectric
method
Regional hemoglobin oxygen saturation: absorption spectrophotometry
Regional RBC and plasma content: radioisotope technique
be evaluated through alkaline phosphatase staining method (
1
),
infusion of fl uorescent dextran or plasma (
2
), and Evans blue
infusion (
3
). However, these techniques are explained in section
dealing with cerebral ischemia in this topic and so they have been
skipped in this chapter.
2. Materials
1. Direct observation of microcirculatory vessel wall as mentioned
in Chapters
46
,
48
,
and
49
.
2. Observation of microvascular architecture.
(a) Casting method.
Polyester resin (Mercox): kept at room temperature.
Slightly harmful for inhalation.
20% NaOH: dissolved before use.
Harmful for contact.
(b) Carbon black injection.
Gelatin: powder. dissolved before use.
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