Biology Reference
In-Depth Information
Table 18.2 Effect of Proto accumulation on
T
.
ni
larval death and body weight change in darkness
and in the light
Post-spray
larval death
after
treatment
Light-dark regime after illumination
treatment
Proto content after
the 17-h post-spray
treatment and 0-6 h
illumination
(protein)
Post-dark
incubation body
weight
per live larva (mg)
17 h
dark
(%)
Total
65-h
(%)
Entry Incubation
0.2
b
A
1
Control, two 14-h light-
10-h dark
photoperiods
1
12
45.1
Treated
a
, 0-h light +
48-h dark
62.0
b
2
28
31
44.5
0.4
c
3
Treated, 3-h light +
45-h dark
21
60
43.4
1.1
d
4
Treated, 6-h light +
42-h dark
22
73
26.6
62.0
b
5
Treated, after two 14-h
light-10-h dark
photoperiod
19
80
16.6
0.2
b
B
1
Control, two 14-h light-
10-h dark
photoperiods
0
22
44.1
Treated
a
, 0-h light +
48-h dark
40.1
b
2
19
48
45.7
1.2
c
3
Treated, 3-h light +
45-h dark
20
65
32.1
0.6
c
4
Treated, 6-h light +
42-h dark
21
77
20.5
40.1
b
5
Treated, after two
14-h light-
10-h dark photoperiod
22
95
3.6
0.808
e
0.815
f
Correlation coefficient
Levels of significance
5 %
1 %
Adapted from Rebeiz et al. (
1988a
)
a
Treatment consisted in spraying larvae with 40 mM ALA + 30 mM Dpy
b
Proto content after the post-spray 17-h dark incubation
c
Proto content after the post-spray 17-h dark incubation and 3 h of illumination
d
Proto content after the post-spray 17-h dark incubation and 6 h of illumination
e
Correlation between larval death and the extent of post-spray exposure to light. In computing the
correlation coefficient, the larval death of the control was subtracted from that of the treated
f
Correlation between the extent of post-spray exposure to light and average body weight change
per surviving larva. In calculating the correlation coefficient, the average body weight of the
control larvae was subtracted from that of the treated larvae
ALA + Dpy. After dark incubation the larvae were analyzed for Proto accumulation,
while duplicate sets of larvae were exposed to light. Treatment with ALA alone or
Dpy alone, resulted in Proto accumulation and concomitant photodynamic death
(Table
18.3
, A). However, treatment with the ALA + Dpy mixture exhibited definite
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