Biology Reference
In-Depth Information
Table 18.3 Synergistic effects of ALA and Dpy on Proto accumulation and larval death in
T
.
ni
a
Proto content
(nmol/100 mg protein)
Larval death after 3 days
in the greenhouse (%)
Experiment Entry Treatment
A
1
Control
0
6
2
40 mM ALA
2
26
3
30 mM Dpy
15
41
4
40 mM ALA
+ 30 mM Dpy
80
90
B
1
Control
0
2
2
30 mM Dpy
11
61
3
30 mM Dpy
+ 10 mM ALA
75
86
4
30 mM Dpy
+ 20 mM ALA
89
76
5
30 mM Dpy
+ 40 mM ALA
73
92
C
1
Control
0
7
2
15 mM Dpy
1
22
3
15 mM Dpy
+ 10 mM ALA
8
42
4
15 mM Dpy
+ 20 mM ALA
34
40
5
15 mM Dpy
+ 40 mM ALA
27
43
D
1
Control
0
5
2
40 mM ALA
1
7
3
40 mM ALA
+ 5 mM Dpy
3
4
7
c
4
40 mM ALA
+ 10 mM Dpy
18
5
40 mM ALA
+ 20 mM Dpy
12
34
6
40 mM ALA
+ 40 mM Dpy
15
71
Correlation between Proto content and larval death
0.857
Level of significance
0.1 %
Adapted from Duggan and Gassman (
1974
)
a
Larvae were in the third instar
synergistic effects, with Proto accumulation (80.4 nmol) and larval death (90 %) far
exceeding the sum of Proto accumulation (2.5 + 15.5
¼
18 nmol) and larval death
(26 + 41
67 %) caused by separate ALA and Dpy treatments (Table
18.3
,A).
The observed levels of Proto accumulation indicated that Dpy was both an inducer and
an enhancer of Proto accumulation. In the absence of added ALA, Dpy caused the
accumulation of Proto over and beyond the level in control larvae, sprayed with
solvent only (Table
18.3
, A3, B2). Under these circumstances, Dpy acted as an inducer
of Proto formation (Rebeiz et al.
1988a
). In the presence of ALA, Dpy enhanced the
conversion of ALA to Proto. This was evidenced by the dramatic increase in Proto
¼
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