Biology Reference
In-Depth Information
Table 18.1 Effect of ALA + Dpy spray on the biosynthesis and accumulation of Proto on the
extent of larval death in
T
.
ni
Proto content after 17 h of dark incubation
(nmol per 100 mg protein)
Larval death
(%)
Experiment
Treatment
A
Control
0.1
3
Treated
85.0
80
Δ
Change
84.9
77
B
Control
0.0
1
Treated
96.7
75
Δ
Change
96.7
74
C
Control
0.1
13
Treated
12.8
47
Δ
Change
12.7
34
D
Control
0.1
6
Treated
89.7
89
Δ
Change
89.6
83
Correlation coefficient 0.945
Level of significance 0.1 %
Larvae were in the third instar. Larval death refers to percent death at the beginning of the fourth
photoperiod, i.e. after 3 days in the growth chamber.
Δ
Change refers to the difference in Proto
content between the ALA + Dpy-treated larvae and the control larvae which were sprayed with
solvent only, after 17-h post-spray dark incubation period (Adapted from Rebeiz et al.
1988a
)
Fig. 18.2 Larval death
in control (
C
) and treated
(40 mM ALA + 30 mM
Dpy) third instar
T
.
ni
larvae
(
D
) 24 h after spraying.
C
control,
D
treated
(Adapted from Rebeiz
et al.
1988a
)
result of photo destruction, a well-known tetrapyrrole phenomenon. Significant
correlations were observed between larval death and the extent of post-spray
exposure to light and between the extent of post-spray exposure to light and
inhibition in body weight gain per surviving larvae (Table
18.2
).
18.2.4 Synergistic Effects of ALA and Dpy on Proto
Accumulation and Larval Death in T. ni
To determine the relative effects of each component of the ALA + Dpy spray on
the insect, third instar
T
.
ni
larvae were sprayed with ALA alone, Dpy alone and
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