Biology Reference
In-Depth Information
Table 3.4 Determination of the reliability of Eq. (
3.35
0
) which was used in the calculation of the Soret excitation amplitude of Ch(lide))
b
at E 460 nm in
mixtures containing Chl
a
, Chl
b
, Pheo
a
and Pheo
b
Soret excitation
fluorescence units
at 460 nm
Percent error between amount
added and amount calculated
(%)
Mean percent error (
X
2
)
standard
deviation (
Sx
2
) of the mean percent
error
Pigments present
Amount of pigments
Chl
a
Chl
b
Pheo
a
Pheo
b
Added Calculated
Mixture 9: picomoles per m/
0
675
0
0
90.8
90.5
0.3
0.3 %
0.1 %
89.1
88.8
0.3
Percentage of total pigment
0
100 % 0
0
88.4
88.1
0.3
86.1
85.7
0.5
89.1
88.9
0.2
Mixture 10: Picomoles per m/ 198
195
178
170
25.6
22.7
11.3
10.0 %
2.6 %
25.6
22.4
12.5
Percentage of total pigment
27 % 26 % 24 % 23 % 25.6
22.9
14.1
25.6
23.5
8.2
25.6
23.5
8.2
Mixture 11: Picomoles per m/ 240
121
243
279
16.0
12.0
24.4
16.0
12.2
23.8
Percentage of total pigment
27 % 14 % 28 % 32 % 16.0
12.8
20.0
23.3 %
2.2 %
16.0
12.0
25.0
Mixture 12: Picomoles per m/ 693
160
815
880
21.1
13.9
34.1
21.1
13.5
36.0
Percentage of total pigment
27 % 6 %
32 % 35 % 21.1
12.0
43.1
38.9 %
3.9 %
21.1 12.2
42.2
21.1 12.8
39.3
The Soret excitation spectra were recorded at a emission maxima of 674 and 660 nm respectively
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