Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 85

Where

K 25 ¼ Chl ð ide Þ a ð F660 E438 Þ= Chl ð ide Þ a ð F660 E460 Þ

K 26 ¼ Pheo ð bide Þ a ð F660 E438 Þ= Pheo ð bide Þ a ð F660 E460 Þ

K 27 ¼ Chl ð ide Þ b ð F660 E438 Þ= Chl ð ide Þ b ð F660 E4I2 Þ

K 28 ¼ ð Þ ð Þ= ð Þ ð Þ

Pheo bide b F660 E438 Pheo bide b F660 E460

K 29 ¼ Chl ð ide Þ a ð F674 E433 Þ= Chl ð ide Þ a ð F660 E460 Þ

K 30 ¼ Pheo ð bide Þ a ð F674 E433 Þ= Pheo ð bide Þ a ð F660 E460 Þ

K 31 ¼ Chl ide ðÞ b F674 E433 ð Þ= Chl ide ðÞ b ð F660 E460

K 32 ¼ Pheo ð bide Þ b ð F674 E433 Þ= Pheo ð bide Þ b ð F660 E460 Þ

K 33 ¼ Chl ð ide Þ a ð F674 E412 Þ= Chl ð ide Þ a ð F60 E460 Þ

K 34 ¼ Pheo ð bide Þ a ð F674 E412 Þ= Pheo ð bide Þ a ð F660 E460 Þ

K 35 ¼ Chl ð ide Þ b ð F674 E412 Þ= Chl ð ide Þ b ð F660 E460 Þ

K 36 ¼ Pheo ð bide Þ b ð F674 E412 Þ= Pheo ð bide Þ b ð F660 E460 Þ

The numerical values of k 25 - k 36 are reported in Table 3.4 .They were calculated
with the above k equations from the same Soret excitation spectra used to calculate
k 1 - k 12 .
Every k value reported in Table 3.4 is the mean of 15-25 different
determinations.
By substituting the numerical values of k 25 -k 36 into the four simultaneous
equations described above and by solving for Chl(ide) b (F660 E460) the following
equation was obtained:

Chl ð ide Þ b ð F660 E460 Þ¼ 0 : 067 ð F674 E433 Þþ 1 : 028 ð F660 E460 Þ

þ : ð Þ : ð Þ

0 057 F660 E460 0 086 F660 E438

: ð Þ ð :

35 0 Þ

0 061 F674 E412 3

3.4.3.2 Determination of the Reliability of Eq. ( 3.35 0 ) and
Determination of Its X 1 and Sx 1 Correction Factors

The reliability of Eq. ( 3.35 0 ) was tested via the following equation:

Chl ð ide Þ b ð F660 E460 Þ¼ ½ A 3 þð A 3 Þð X 3 Þ= 100 ð A 3 Þð S X3 Þ= 100 (3.35)

Where:
A 3 ¼ Eq. ( 3.35 )

[(A 3 )(X 3 )/100] are relative concentration-correction factors, which vanish as

the X values approach zero. The X 3 correction values are listed in Table 3.4 and
their derivation and usage is as for Eq. ( 3.33 ). Finally, the last term in Eqs. ( 3.35 ),
i.e . (A 3 ) (Sx 3 )/100 refers to the standard deviation (Sx 3 ) of the determinations
expressed as a percentage of the pigment content. The Sx 3 values are also listed
in Tables 3.4 and their derivation is as discussed for Eq. ( 3.33 ).
The determination of the X 3 and S x 3 correction values for various relative
concentrations Chl(ide) B are reported in Table 3.4 . The mean per cent error (X 3 )

¼