Biomedical Engineering Reference
In-Depth Information
15.
Appl Microbiol Biotechnol
. 2010
Mar;86(1):41-9. Epub 2010 Jan 22.
Disposable bioreactors: the
current state-of-the-art and
recommended applications in
biotechnology. Eibl R, Kaiser S,
Lombriser R, Eibl D. School of
Life Sciences and Facility
Management, Institute of
Biotechnology, Zurich University
of Applied Sciences, P.O. Box,
CH-8820, Wädenswil,
Switzerland.
regine.eibl@zhaw.ch
Disposable bioreactors have increasingly been
incorporated into preclinical, clinical, and
production-scale biotechnological facilities over the
last few years. Driven by market needs, and, in
particular, by the developers and manufacturers of
drugs, vaccines, and further biologicals, there has
been a trend toward the use of disposable seed
bioreactors as well as production bioreactors.
Numerous studies documenting their advantages in
use have contributed to further new developments
and have resulted in the availability of a multitude of
disposable bioreactor types that differ in power
input, design, instrumentation, and scale of the
cultivation container. In this review, the term
disposable bioreactor
is defined, the benefits and
constraints of disposable bioreactors are discussed,
and critical phases and milestones in the
development of disposable bioreactors are
summarized. An overview of the disposable
bioreactors that are currently commercially available
is provided, and the domination of wave-mixed,
orbitally shaken, and, in particular, stirred disposable
bioreactors in animal-cell-derived productions at
cubic meter scale is reported. The growth of this type
of reactor system is attributed to the recent
availability of stirred disposable benchtop systems
such as the Mobius CellReady 3 L Bioreactor.
Analysis of the data from computational fluid
dynamic simulation studies and first cultivation runs
confirms that this novel bioreactor system is a viable
alternative to traditional cell culture bioreactors at
benchtop scale.
16. Bioresour Technol. 2010
Apr;101(8):2896-9. Epub 2009
Dec 31. Study on
poly-hydroxyalkanoate (PHA)
production in pilot scale
continuous mode wastewater
treatment system. Chakravarty P,
Mhaisalkar V, Chakrabarti T. Ion
Exchange Waterleau Ltd.,
Process and Proposal Division,
Reveira Apartments, 4th Floor,
Plot No. 134, 6-3-347/9
Punjagutta, Hyderabad 500 082,
India.
partha_chakravarty@
Generation of poly-hydroxyalkanoates (PHAs) from
milk and ice-cream processing wastewater was
studied in a continuous mode reactor system at pilot
scale. The integrated system comprised an anaerobic
acidogenic reactor (AAR), a conventional activated
sludge production reactor (ASPR), and a PHA
synthesis reactor (PHAR) to induce PHA
accumulation in the biomass, which was finally
harvested while treating the raw dairy wastewater to
meet the disposal limits, thereby reducing generation
of disposable sludge. The PHA content in the PHA
rich biomass was approximately 43% of the sludge
dry weight. Kinetics of both ASPR and PHAR were
studied. The maximum PHA yield coefficient (Y(sp)
(max)) with respect to COD degradation in the
PHAR was derived as 0.25 kg PHA/kg of COD
degraded. Similarly, the kinetic parameters, that is,
K(s), micro(m), Y(obs), and k(d) of the ASPR were
37.16 mg/L COD, 0.97 d(−1), 0.51 mg MLSS/mg
COD, and 0.049 d(−1), respectively.
