Biomedical Engineering Reference
In-Depth Information
13.
Bioprocess Biosyst Eng
. 2010 Oct;
33(8):961-70. Epub 2010 Mar 27.
A BOD monitoring disposable
reactor with alginate-entrapped
bacteria. Villalobos P, Acevedo
CA, Albornoz F, Sánchez E,
Valdés E, Galindo R, Young ME.
Centro de Biotecnología,
Universidad Técnica Federico
Santa María, Avenida España
1680, Valparaíso, Chile.
patricio.
Biochemical oxygen demand (BOD) is a measure of
the amount of dissolved oxygen that is required for
the biochemical oxidation of the organic compounds
in 5 days. New biosensor-based methods have been
conducted for a faster determination of BOD. In this
study, a mathematical model to evaluate the
feasibility of using a BOD sensor, based on
disposable alginate-entrapped bacteria, for
monitoring BOD in situ was applied. The model
considers the influences of alginate bead size and
bacterial concentration. The disposable biosensor can
be adapted according to specific requirements
depending on the organic load contained in the
wastewater. Using Klein and Washausen parameter
in a Lineweaver-Burk plot, the glucose diffusivity
was calculated in 6.4 × 10(−10) (m
2
/s) for beads 1
mm in diameter, and slight diffusion restrictions
were observed (
n
= 0.85). Experimental results
showed a correlation (
p
< 0.05) between the
respirometric peak and the standard BOD test. The
biosensor response was representative of BOD.
14.
Protein Pept Lett
. 2010
Jul;17(7):919-24. Transient
expression of recombinant
sPDGFR alpha-Fc in CHO DG44
cells using 50-mL orbitally
shaking disposable bioreactors.
Sang YX, Zhang XW, Chen XJ,
Xie K, Qian CW, Hong A, Xie
QL, Xiong S. Biomedical R&D
Center, Guangdong Provincial
Key Laboratory of
Bioengineering Medicine,
National Engineering Research
Center of Genetic Medicine,
Jinan University, Guangzhou
510632, Guangdong, PR China.
Overactivity of platelet-derived growth factor (PDGF)
has been linked to malignant cancers. High levels of
PDGF result in the activation of its receptors
(PDGFRs) and the overproliferation of cells.
Therefore, interfering with this signaling pathway in
cancer cells could be significant for anticancer drug
development. In a previous study, the sPDGFR
alpha-Fc fusion protein expressed in static CHO-k(1)
cells showed an antiproliferative effect on vascular
endothelial cells. However, it was difficult to obtain a
large quantity of this fusion protein for further
functional studies. In the present study, the sPDGFR
alpha-Fc fusion protein was transiently expressed in
Chinese Hamster Ovary (CHO) DG44 cells in 50 mL
orbital shaking bioreactors. sPDGFR alpha-Fc was
expressed as a 250 kDa dimeric protein with potential
glycosylation. The final yield of sPDGFR alpha-Fc in
the culture supernatant was as high as 16.68 mg/L.
Our results suggest that transient expression in
orbital shaking bioreactors may be feasible for
preparation of recombinant proteins used for
preclinical studies.
