Biomedical Engineering Reference
In-Depth Information
17.
Biotechnol Prog
. 2010 Mar;
26(2):332-51. Technological
progresses in monoclonal
antibody production systems.
Rodrigues ME, Costa AR,
Henriques M, Azeredo J, Oliveira
R. IBB-Institute for
Biotechnology and
Bioengineering, Centre of
Biological Engineering,
University of Minho, Campus de
Gualtar 4710-057 Braga,
Portugal.
Monoclonal antibodies (mAbs) have become vitally
important to modern medicine and are currently one
of the major biopharmaceutical products in
development. However, the high clinical dose
requirements of mAbs demand a greater
biomanufacturing capacity, leading to the
development of new technologies for their large-
scale production, with mammalian cell culture
dominating the scenario. Although some companies
have tried to meet these demands by creating
bioreactors of increased capacity, the optimization of
cell culture productivity in normal bioreactors
appears to be a better strategy. This review describes
the main technological progress made with this idea,
presenting the advantages and limitations of each
production system, as well as suggestions for
improvements. New and upgraded bioreactors have
emerged both for adherent and suspension cell
culture, with disposable reactors attracting increased
interest in the last few years. Furthermore, the
strategies and technologies used to control culture
parameters are in constant evolution, aiming at the
online multiparameter monitoring and considering
how parameters were not seen as relevant for process
optimization in the past. All progress being made has
as primary goal the development of highly
productive and economic mAb manufacturing
processes that will allow the rapid introduction of
the product in the biopharmaceutical market at more
accessible prices.
18.
Appl Microbiol Biotechnol
. 2010
Feb;85(5):1339-51. Epub 2009
Dec 3. Bench to batch: advances
in plant cell culture for
producing useful products.
Weathers PJ, Towler MJ, Xu J.
Department of Biology and
Biotechnology at Gateway,
Worcester Polytechnic Institute,
Worcester, MA 01609.
weathers@
Despite significant efforts over nearly 30 years, only a
few products produced by in vitro plant cultures
have been commercialized. Some new advances in
culture methods and metabolic biochemistry have
improved the useful potential of plant cell cultures.
This review will provide references to recent relevant
reviews along with a critical analysis of the latest
improvements in plant cell culture, co-cultures, and
disposable reactors for production of small
secondary product molecules, transgenic proteins,
and other products. Some case studies for specific
products or production systems are used to illustrate
principles.
