Electrochemical Transducer for Oligonucleotide Biosensor Based on the Elimination and Adsorptive Transfer Techniques - Electrochemical DNA Biosensors - page 366

Figure 11.4. LSV and EVLS voltammograms of three hetero-ODN: 5'-
CCCAAACCC-3'(red),5'-CACCACCAC-3'(blue),and5'-ACCCACCCA-3'(green)
in phosphate buffer (pH 6.2). f ( I ): EVLS E4 for simultaneous elimination
of kinetic and charging currents, and conserving the diffusion current.
Scan rates for EVLS: 100, 200, 400 mV/s, reference scan 200 mV/s,
potential step 2 mV, time of accumulation 90 s at 100 mV vs. Ag/AgCl/3M

KCl. Reproduce with permission from Trnkova, L., et al. , Application of
eliminationvoltammetrytotheresolutionofadenineandcytosinesignalsin
oligonucleotides II. Hetero-oligodeoxynucleotides with different sequences
of adenine and cytosine nucleotides, Electroanalysis 18 , 662 (2006).
Copyright Wiley-VCHVerlag GmbH& Co. KGaA. See also Color Insert.

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Our results showed that EVLS in connection with the adsorption
procedure (adsorptive or adsorptive transfer stripping, i.e., AdS or
AdTS) is a useful tool for qualitative and quantitative studies of
short oligonucleotides and can be used as a proposed transducer
fortheelectrochemicalsensor.EVLSsensitivelyreflectsnotonlythe
sequence of nucleobases in the ODN chain, but also the structure of
ODN, which can bechanged on electrode surfaces.

11.4.2 AdS EVLS of Hairpins
Hairpin structures in DNA and RNA consisting of stem and loop
regions occur naturally not only in ssDNAs and RNAs but also in
double-stranded DNAs (dsDNAs), and they have an important role
in many biological processes. They play a major role in expansion
events, mainly in the case of triplet-repeated expansion diseases
(X syndrome, Huntington disease, Friedreich ataxia). The short
fragment d(GCGAAGC) has been found in the replication origins of
phage

φ

X 174 and herpes simplex virus, in a promoter region of an