Biology Reference
In-Depth Information
Figure 11.3.
LSV and EVLS voltammograms of hetero-ODN
5'-CCCAAACCC-3' in phosphate buffer (pH 6.2).
f
(
I
): elimination function
E4 for simultaneous elimination of kinetic and charging currents, and
conserving the diffusion current. Scan rates for EVLS: 100, 200, 400 mV/s,
reference scan 200 mV/s, potential step 2 mV, and time of accumulation
90s at -100 mV vs. Ag/AgCl/3M KCl. Reproduced with permission
from Trnkova, L.,
et al.
, Application of elimination voltammetry to the
resolution of adenine and cytosine signals in oligonucleotides II. Hetero-
oligodeoxynucleotides with different sequences of adenine and cytosine
nucleotides,
Electroanalysis
18,
662 (2006). Copyright Wiley-VCH Verlag
GmbH& Co.KGaA.
It was found that the AdS EVLS is capable of reflecting
small differences in the sequences and of distinguishing adjacent
and nonadjacent bases in the ODN chain. Depending on pH the
substantial changes in EVLS signals were observed in the case of
ODN containing a triplet of As and Cs. Alternating A and C in
ODN chains has resulted in weakening of noncovalent interactions
(i-motif) and in decreasing of efforts to form a chain of ODN
multiplexes. The worse separation of A and C signals can indicate
that ODN chain containsA atits end (Fig. 11.4).
As shown in Fig. 11.4, EVLS sensitively reflects the change in
sequence of the ODN chain. Moreover, the EVLS peak-counterpeak
signalisabout 5times higher than the originalLSV signal.
