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Escherichia coli heat-shockgene,andinrRNAgenes.Exceptspectral
and thermodynamic analysis (CD, NMR, and calorimetry), this
heptamer was studied electrochemically (CV, LSV, EVLS) [46]. On
mercuryelectrodesthehairpind(GCGAAGC)providesvoltammetric
reduction signals of A and C, and oxidation signals of G. Both signals
have been studied in dependence on pH, accumulation time, scan
rate, and loop sequences. The AdS EVLS was employed for the
determination of the detection limit (2 nM), which was verified
bymultidimensionalvoltammetricanalysisusingFouriertransform
in combination with the confidence ellipse statistic method. Our
results showed the difference in electrochemical behavior of DNA
and RNA heptamers (Fig. 11.5).
WhileRNA hairpin (Fig. 11.5b) provides oneanodicGpeak, DNA
hairpin gives two G peaks (Fig. 11.5a) whose heights depend on pH.
This phenomenon is very interesting because guanine-containing
compounds on mercury electrodes provide a single anodic peak G,
which corresponds to the oxidation of reduction product generated
(a)
(b)
Figure 11.5. Application of AdS EVLS in the research of DNA and
RNA hairpins (5'-GCGAAGC-3'). LSV and EVLS of anodic signal of G in
(a) heptamer DNA and (b) heptamer RNA in a concentration of 1 μ M
(phosphate-acetate buffer, pH 5.3). LSV parameters: scan rate 200 mV/s,
potential step 2 mV, accumulation time 90 s, and time of accumulation 90
sat 100 mV vs. Ag/AgCl/3M KCl. EVLS E4 utilized three scan rates: 100,
200, and 400 mV/s.
 
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