Biology Reference
In-Depth Information
Table 1.7 Types of supportive cells used to enhance chitosan tubes in peripheral nerve repair
Combination with cells
Gap
size
(mm)
Methods
Authors
and year
Nerve tube
Cell type
Internal filler
Animal
Nerve
Injury
Controls
Follow-up
Outcome
Silicon tube
Autologous bone
marrow
mesenchymal
stem cells
(BMSCs)
Laminin-
modified
chitosan film
with BMSCs
Female
Sprague-
Dawley
rats
Sciatic
nerve
Nerve
transection
10
Histological
evaluation and
FluoroGold
retrograde tracing
Empty silicon tube
16 weeks
BMSCs containing
scaffolds improve
nerve regrowth,
muscle mass
maintenance, and
functional recovery.
Hsu
et al.
(2013)
Chitosan tube
Autologous bone
marrow
mesenchymal
stem cells
(BMSCs)
Longitudinally
aligned poly
lactic-
co
-
glycolic acid
(PLGA) fibers
Rhesus
monkeys
Median
nerve
Nerve
transection
50
Electrophysiological
and histological
evaluation,
FluoroGold
retrograde tracing
Chitosan-PLGA
fibers alone
12 months Twelve months after
grafting, nerve
function recovery,
and morphological
reconstruction of
BMSCs containing
PLGA/chitosan
scaffold is superior to
that of chitosan/
PLGA scaffold alone
Hu
et al.
(2013)
Chitosan tube
Autologous bone
marrow
mesenchymal
stem cells
(BMSCs)
Poly lactic-
co
-
glycolic-acid
(PLGA)
oriented fibers
Male
Beagle
dogs
Sciatic
nerve
Nerve
transection
60
Electrophysiological,
retrograde fluorogold
tracing, and
histological
evaluation
Autograft and
chitosan/PLGA
fibers alone
12 months MSCs in
combination with
chitosan/PLGA fibers
tube improve nerve
repair in comparison
to chitosan/PLGA
fibers tube alone
Xue
et al.
(2012)
Chitosan tube
Bone marrow
stromal cells
(BMSCs)-derived
Schwann cells or
sciatic nerve-
derived Schwann
cells (SCs)
-
Rats
Sciatic
nerve
Nerve
transection
8
Sciatic nerve function
index (SFI) and
histological
evaluation
Chitosan tube alone 6 weeks
Six weeks
postsurgery, the SFI,
average regenerated
fiber density, and
fiber diameter in
nerve bridged with
BMSCs are similar to
autograft
Zheng
and Cui
(2012)
