Biology Reference
In-Depth Information
Gap
size
(mm)
Methods
Authors
and year
Nerve tube
Cell type
Internal filler
Animal
Nerve
Injury
Controls
Follow-up
Outcome
Chitosan tube
Bone marrow
stromal cells
(BMSCs)-derived
Schwann cells or
sciatic nerve-
derived Schwann
cells (SCs)
Matrigel
Male
Sprague-
Dawley
rats
Sciatic
nerve
Nerve
transection
12
Footprint analysis,
compound muscle
action potential
(CMAP)
measurements,
histological
evaluation
Autograft and PBS-
filled conduits
3 months Nerve conduction
velocity, average
regenerated myelin
area, and myelinated
axon count in nerve
bridged with
BMSCs-derived SCs
are similar to those
treated with sciatic
nerve-derived SCs
and higher than those
bridged with PBS-
filled conduits
Ao et al.
(2011)
Chitosan/polyglycolic acid tube
Autologous bone
marrow
mesenchymal
stem cells
(BMSCs)
-
Beagle
dogs
Sciatic
nerve
Nerve
transection
50
Electrophysiological
and histological
evaluation,
FluoroGold
retrograde tracing
Autograft
6 months
Introduction of
BMSCs in the
conduits promotes
nerve regeneration
and functional
recovery
Ding
et al.
(2010)
Chitosan-3-
glycidoxypropyltrimethoxysilane
(GPTMS) cross-linked
membranes
Predifferentiated
N1E-115 cells
Chitosan
membrane
covered with a
cell monolayer
Female
Wistar
rats
Sciatic
nerve
Crush
injury
10
Kinematic analysis
and histological
evaluation
Unoperated
animals
8 weeks
Local enwrapping
with chitosan
membrane without
N1E-115 cells
improves axonal
regrowth and
functional recovery
Amado
et al.
(2008)
Chitosan-Au-nanocomposites
Neural stem cell
(NSC)
-
Male
Sprague-
Dawley
rats
Sciatic
nerve
Nerve
transection
10
Histological
evaluation
Chitosan-
Aunanocomposites
tube without NSC
6 weeks
In comparison to
control group, the
number of
regenerated axons,
the regenerated area,
and the number of
blood vessels are
significantly higher in
the NSCs preseeded
Lin,
Jen,
Hsu,
and
Chiu
(2008)
