Biomedical Engineering Reference
In-Depth Information
6 nm
100 nm
Figure 16.3.
Transmission electron micrograph of a biomimetic self-assembly nano-HA/
collagen composite.
73
TABLE 16.3. Biomimetic Nano - HA /Collagen - based Composite for Bone Tissue Engineering
Nanocomposite
Cell/Growth factors
Tissue reactions
References
Nano - HA/Collagen
Osteogenic cells
(
In vitro
and
in vivo
)
In a marrow cavity or
bone fragment co-
culture, interfacial bone
formation at two weeks.
Refs
77,84,85
Nano - HA/Collagen
Without/with BMP - 2
(
In vivo
)
Resorbed by
phagocytosis of
osteoclast - like cells and
form new bone in the
surrounding area at 12
weeks.
Refs
75,76,81
Nano - HA/Collagen
Chondroitin sulfate
No
Refs
74,80,83
Nano - HA/
Collagen/PLA
Osteoblasts (
In vitro
)
In - growth 400 μ m depth
of the porous scaffold.
Ref
79
Nano - HA/
Collagen/PLA
rhBMP - 2 (
In vivo
)
8 weeks complete bone
defect repair.
Refs
72,78
Nano - carbonated
HA/Collagen/
PLGA
Osteoblasts (
In vitro
)
(
In vivo
)
Enhance osteoblast and
bone regeneration.
Refs
70,86
Ref
82
Nano - HA/
Collagen/Alginate
Fibroblasts/Osteoblasts
(
In vitro
)
Positive affect on
osteoblasts.
made in the radius of 24 rabbits and the graft material was inserted into the defect
with the incorporation of 0.5 mg of recombinant human bone morphogenetic
protein-2 (rhBMP-2). Observations were made at 4, 8, 12 and 16 weeks, with six
samples at each time point. Figure 16.4a shows the X-ray observation of the defect
immediately after implantation. Figure 16.4b shows the X-ray result after 12 week
of implantation where double cortical bone connection was formed. Histological
Search WWH ::
Custom Search