Aberrations and the Benefit of Their Correction in Confocal Microscopy - Adaptive Optics for Biological Imaging - page 62

(a) (b) (c)

Figure 4.9 (See color insert.) Examples for measured complex pupil plane wavefronts. he color represents the
(wrapped) phase, while the brightness corresponds to the recorded intensity. (a) Wavefront recorded beside the cell
(objective NA = 0.5, dry, condensor NA = 0.6). (b) Aberrating region of the sample (mouse blastocyst, objective
NA = 0.5, dry, condensor NA = 0.6). (c) Aberrating region of the sample (mouse oocyte, objective NA = 0.8, oil,
condensor NA = 0.6). he circles indicate the limiting aperture of the system.

Total Zernike Mode 2—Tip Mode 3—Tilt

2

3

2

1

1

0
̶ 1
̶ 2
̶ 3

0

Mode 4—Defocus Mode 5—Astigmatism Mode 6—Astigmatism Mode 7—Coma

1

0.5
0

̶ 0.5

̶ 1

Mode 8—Coma Mode 9 Mode 10 Mode 11—Spherical (1st) Mode 12

Mode 13 Mode 14 Mode 15 Mode 16 Mode 17

Mode 18 Mode 19 Mode 20 Mode 21 Mode 22—Spherical (2nd)

Figure 4.10 (See color insert.) Zernike mode plots of the mouse oocyte sample, coeicients 2-22. For these images,
the Zernike modal content was extracted from 256 wavefronts. Objective lens: Zeiss Plan-Neoluar 20×, 0.5 NA, dry
lens. Condenser lens: Zeiss LD-Achroplan, 40×, 0.6 NA, correction ring. he scanned area was 100 × 100 μm.