Biomedical Engineering Reference
In-Depth Information
Fig. 2.8
UV-vis absorption spectra and transmission electron micrographs of AuNR-protein
complexes before and after laser irradiation or thermal treatment at 45
C for 55 min. (
a
) UV-vis
spectra for 10 % protein-AuNR complexes following hyperthermia treatment. Green spectrum:
45
C treatment. Blue spectrum: 37
C treatment. Black spectrum: 27 min laser irradiation. Red
spectrum: 55 min laser irradiation. (
b
) UV-vis spectra for 100 % protein-AuNR complexes
following treatment. (
c
,
d
) TEM images of CTAB-AuNRs (scale bars 100 nm, 50 nm, respec-
tively). (
e
) TEM image of a protein-AuNR complex (10 % FBS, scale bar 20 nm). (
f
) TEM image
of protein-AuNR complex (100 % FBS, scale bar 25 nm) (adapted with permission from [
34
])
proteins are presented in Tables
2.2
and
2.3
. It is notable that the total number of the
mass spectroscopy values of the peptides can be analyzed using semiquantitative
analysis of the amount of proteins through application of spectral counting method
(SpC). The normalized SpC amounts of each protein, identified in the mass
spectroscopy study of nanoparticles, would be calculated by applying the following
equation:
0
1
SpC
ðM
w
Þ
k
@
A
NpSpC
k
¼
100
(2.3)
P
i¼
1
SpC
ðM
w
Þ
i
where NpSpC
k
is the normalized percentage of spectral count for protein k, SpC is
the spectral count identified, and
M
w
is the molecular weight (in kDa) of the protein
k. According to the results, one can find that the hyperthermia treatments had
modest effects on the overall surface charge of the protein corona associated with
the gold nanorods following irradiation but that there were significant changes in
the composition of the hard protein corona following irradiation,
including
