SHG Imaging for Tissue Engineering Applications - Second Harmonic Generation Imaging - page 436

(a) (b) (c)

80 μm 80 μm 80 μm

LC

IE

L

L

CARS SFG TPEF

(d) (e) (f )

300 μm 300 μm 300 μm

LC

LC

L L

(g) (h) (i)

30 μm 30 μm 30 μm

Dox. labeled

Figure 13.10 Representative images of a Type IV lesion inspected by CARS (a), SFG (b), and TPEF (c). The lipid
core (LC) was identified by CARS. (d and e) Masson's trichrome staining. Black rectangle: the corresponding area
in (a) through (c). (f) H&E staining. (g and h) Colocalized NLO images corresponding to the red and yellow squares
in (a), respectively. (i) Doxorubicin-labeled (red) image, around lipid core, colocalized with CARS and SFG signals.
(Reproduced from Wang, H. W. et al. 2009. Arterioscl Throm Vas 29:1342-1348. With the permission of SPIE.)

Figure 14.4 In vivo 3D images of (a) a zebrafish heart and (b)-(c) a zebrafish brain. The 3D images are
reconstructed from stacks of HGM images versus depth. (a) The 3D structures of the cardiac muscles (SHG; green),
cardiac cells (2PF; red), and red blood cells (THG; yellow); and (b) the 3D structures of the neural tube (THG;
purple), otic vesicles (arrows; THG; purple), and the nerve fibers (SHG; green) can be observed with a submicron
resolution. Image size: 240 × 240 μm 2 .