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Figure 14.6
See main text for figure caption.
Figure 15.2 SHG/2PEF imaging of lung fibrosis in bleomycin-treated mice. SHG (green: collagen) and 2PEF
(red: elastin, macrophages) signals excited with 50 mW excitation power at 860 nm. Yellow color: SHG/2PEF colo-
calization. (a) Fresh unlabeled fibrotic lung (day 14), at 42 μm under the pleura, evidencing the heterogeneous
fibrosis distribution; (b) 3D reconstruction of the SHG signal from the underlined area in (a), within the tissue (b1)
and in the pleurae (b2); (c) unstained histological sections embedded in paraffin, from (c1) control, (c2) day 3, (c3)
day 7, and (c4) day 14 bleomycin lungs. Bleomycin treatment induces (i) inflammation, which decreases after day 7,
as evidenced by the 2PEF signal, and (ii) fibrosis, evidenced by the SHG signal detected as early as day 3. Scale bar:
100 μm. (From Pena, A.-M. et al. Three-dimensional investigation and scoring of extracellular matrix remodeling
during lung fibrosis using multiphoton microscopy. Microsc. Res. Tech. 2007. 70:162-170. Copyright Wiley-VCH
Verlag GmbH & Co. KGaA. Reproduced with permission.)
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