INNOVATIVE BIOINSPIRED SIC CERAMICS FROM VEGETABLE RESOURCES - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 62

Figure 2.6. CLSMimagesofpreosteoblasticcelllineMC3T3-E1growthon
sapelli-based SiC ceramics along the time of culture. (a, c) For 1 day; (d)
for 3 days; (b, e, f) for 7 days. Alexa Fluor 488 Phalloidin solution was the
antibodyusedtovisualizethecellcytoskeletonfilamentousactin,orF-actin,
(green) and propidium iodide solution to label cell nuclei (red). See also
Color Insert.
properly, without any signs of cytotoxicity, maintaining the cell-to-
cell and cell-to-materialcontact by filopodia andlamellipodia.
Besidesthecelladhesionandmorphology,thecytoskeletonorga-
nization was also analyzed by CLSM (Fig. 2.6). F-actin filaments and
the progressive covering of the SiC ceramics surface was observed,
proving the tested material to be cell friendly. Cytoskeleton organi-
zation was progressively higher throughout the time of culture, as
shown by the increasing densification of actin filaments, observed
after seven days of incubation (Fig. 2.6b-f). After one day of cell
culture (Fig. 2.6a,c), images showed oval nuclei and some evidence
of mitosis, which means that cells were already spreading over
the SiC surface. At three days of cell culture (Fig. 2.6d), the nuclei
of cells and F-actin fibers are clearly visible. After seven days of
incubation, cells displayed an organization with numerous cell-
to-cell contacts and presented a complex network of actin fibers
(Fig. 2.6e,f).