INNOVATIVE BIOINSPIRED SIC CERAMICS FROM VEGETABLE RESOURCES - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 61

Figure 2.4. Cell proliferation results after incubation of MC3T3-E1 cells
up to 21 days on SiC ceramics and TCP as control. (Statistical significant
differences: ∗ p < 0 . 05, ∗∗ p < 0 . 01 and ∗∗∗ p < 0 . 005).
healthy osteoblasts. After three days of incubation (Fig. 2.5b,e), the
cells, connected to each other by filopodia and lamellipodia, almost
covered the whole surface, including some of the channels. At day
7 of incubation (Fig. 2.5c,f), several layers of cells covered the sur-
face and the channels were almost completely filled. Micrographs
showedahealthyappearanceofthecells,whichattachedandspread
Figure 2.5. SEM images showing the attachment and spreading of the
MC3T3-E1 cell line on bioinspired ceramics. (a, d) For 1 day; (b, e) for 3
days; and (c, f) for 7 days. Two magnifications are shown: (a, b, c) 100x and
(d,e, f)500x.