Biomedical Engineering Reference
In-Depth Information
transplantedcells.
13
Thematerialactsinstantaneously,therebypre-
venting cell loss. Fibrin glue is considered to be biocompatible and
biodegradable because of a physiological component, commercially
available.
14
Another advantage to use fibrin is that it can dissolve
overtimeduetofibrinolysis.
15
Herewecarriedoutastudytoinves-
tigatetheabilityofboneformationwithuseofhumanBMstemcells,
PRP,andafibringluemixture.Wethenevaluatedthepotentialoffib-
rin scaffolds
in vivo
for boneregeneration.
Eighteen New Zealand white rabbits (male, 2,500-3,000 g) were
used in this study. A 6 mm diameter, 6 mm deep critical-sized
defect was created on one tibia of each rabbit.
16
The rabbits were
randomly divided into three groups. In the first group (defect), the
defect in the tibia was left empty. In the second group (fibrin glue
scaffold only), the contained defects were first made on the tibias
and then manufactured scaffolds were implanted into the defects.
Inthethirdgroup(fibrin/humanBMstemcellscompositescaffold),
the contained defects were created on the tibias, as described, and
thenpreparedfibrin/humanBMstemcellscompositescaffoldswere
implanted into the defects.
17
−
19
The skin was sutured with 3/0 silk
sutures. To evaluate the viability of human BM stem cells in a fib-
rin glue scaffold, live/dead staining was carried out at two, four,
and six hours, which stained the live cells in green fluorescence and
dead cells in red fluorescence (Fig. 43.10). There were no statisti-
cally significant differences in cell survival six hours after making
a fibrin/marrow stem cells composite scaffold. The viability of BM
Figure 43.10.
Cell viability was tested using live/dead kit at 2, 4 and 6
hours after a fibrin/human BM stem cells composite scaffold. The human
BMstemcellswereseparatedintotwogroups:livecellsshowedgreenfluo-
rescence and dead cells showed red fluorescence. See also Color Insert.
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