Biomedical Engineering Reference
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Figure 43.11. The viability of the human BM stem cells in a fibrin glue
scaffold. The viability of human BM stem cells in a fibrin glue scaffold was
measured 99.2 ± 0.5% at 6 hours (97.9 ± 1.7 at 2 hours; 97.3 ± 2.2 at 4
hours; 99.2
±
0.5 at 6 hours). See also Color Insert.
stem cells in a fibrin glue scaffold was measured up to 90% average
for six hours(Fig. 43.11).
To assay the osteogenic differentiation potential in vitro ,a
human BM stem cells/fibrin composite scaffold was cultured in an
osteogenic medium for three weeks. Osteogenic differentiation in a
sectionofthefibrin/BMstemcellscompositescaffoldwasobserved
(Fig. 43.12a). Calcium deposition followed by osteogenic differen-
tiation appeared (Fig. 43.12a). Also, osteogenic differentiation in
the fibrin/human BM stem cells scaffold could be demonstrated by
von Kossa staining (Fig. 43.12b). In this study, a fibrin glue scaffold
provided a suitable environment for differentiation of osteoblasts
in vitro .
Bone regeneration at the tibia defect site was assessed using
X-rays 2, 4, 8, and 12 weeks after surgery (Fig. 43.13). X-ray eval-
uation showed there was no difference between the scaffold and
the control group, comparing the degree of mineralization corre-
sponding locations as well as the overall bone regeneration in the
defects during 4, 8, and 12 weeks. 8 , 20 Judging from a macroscopic
view, macroscopic examination of the tibia was performed at 4, 8,
and 12 weeks (Fig. 43.14). All groups of bone defect were observed
in the normal condition. New bone formation was not observed in
the bottom of the defect. 21 And tissue adhesion occurred surround-
ing the tissue of the surgery regions. However the regions of adhe-
sion were reduced gradually as time went by. H&E staining at 12
 
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