POROUS POLY(LACTIC-CO-GLYCOLIC ACID) MICROSPHERE AS CELL CULTURE SUBSTRATE AND CELL TRANSPLANTATION VEHICLE - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 359

Figure 18.2. The number of human ASCs growing on macroporous PLGA
microspheres(square)ornonporousPLGAmicrospheres(circle)inspinner
flask culture. The cell inoculum density was 5 × 10 5 cells/mL.
Human ASCs were isolated from adipose tissue obtained by
liposuction from informed and consenting patients using an enzy-
matic digestive process. The human ASCs were cultured in macrop-
orous PLGA microspheres with a growth medium in spinner flasks
for one week. One day after culture, 78.8 ± 6.0% of the inoculated
ASCs adhered to porous microspheres, while only 36.8 ± 8.0% of the
inoculated ASCs adhered to nonporous microspheres (Fig. 18.2),
as determined according to DNA content measurement. ASCs cul-
tured on macroporous PLGA microspheres in stirred suspension
bioreactors expanded to a large number of cells. The number of
ASCs cultured on the macroporous microspheres and nonporous
microspheres increased 3.8- and 3.7-fold, respectively, over seven
days (Fig. 18.2). Scanning electron microscopic examination of
macroporousPLGAmicrospheresseededwithhumanASCsandcul-
tured in spinner flasks for seven days revealed that ASCs adhered
well on the microsphere surface (Fig. 18.3a). Confocal microscopic
examination revealed that cells were growing on pores throughout
the macroporous microspheres (Fig. 18.3b).