POROUS POLY(LACTIC-CO-GLYCOLIC ACID) MICROSPHERE AS CELL CULTURE SUBSTRATE AND CELL TRANSPLANTATION VEHICLE - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 360

Figure 18.3. Human ASCs growing on macroporous PLGA microspheres
on day 7 in spinner flask culture. (a) Scanning electron microscopic image
and (b) confocal micrograph of DAPI staining of human ASCs cultured
onmacroporousPLGAmicrospheres. Abbreviation :DAPI,4'-6-diamidino-2-
phenylindole. See also Color Insert.
ASCs cultured on macroporous PLGA microspheres have the
capacity to differentiate into mature adipocytes. ASCs were cul-
turedonmacroporousPLGAmicrosphereswithagrowthmediumin
spinnerflasksfor7daysandwerethenculturedwiththeadipogenic
differentiation medium for 10 days. The cells differentiated into an
adipogeniclineage,asindicatedbythecellsstainingpositivelyforoil
red O (Fig. 18.4).
ASCs cultured on macroporous PLGA microspheres had lower
apoptotic activity than trypsinized ASCs. ASCs were cultured on
plates or macroporous microspheres with growth medium for 7
days and subsequently with adipogenic differentiation medium for
Figure 18.4. Human ASCs cultured on a macroporous PLGA microsphere
and adipogenically differentiated for 10 days; oil red O staining. See also
Color Insert.