Biomedical Engineering Reference
In-Depth Information
Figure 18.1.
Scanning electron micrographs of (a) gross morphology and
(b)cross section of open macroporous PLGA microspheres.
(Fig. 18.1a) on the microsphere surface and interconnected struc-
tures between the inner pores (Fig. 18.1b). The pore morphology
in the cross section was similar to that on the surface, indicating
that the porous structure was produced homogeneously through-
out the microspheres. The average diameter of the microspheres
was372
±
43mm.Theaverageporesizeonthemicrosphere surface
6.9mm,whichissu
cientforcellinfiltrationandseeding
within the microspheres.
±
was36.1
18.3 Macroporous PLGA Microsphere as an
ASC Culture Substrate
A microcarrier provides a large surface area for cell attachment
and growth. Microcarrier culture combined with 3D suspension
bioreactors overcomes the limitation of cell growth area in the 2D
culture plate and thus would be suitable for clinical-scale produc-
tion of transplantable cells. A macroporous microsphere with well-
interconnectedporesprovidesalargersurfaceareaforcelladhesion
and growth than a nonporous microsphere, and a larger number of
cells can be expanded in a 3D suspension bioreactor. Furthermore,
openmacroporousmicrospheresfacilitatethetransportofnutrients
and oxygen through the interconnected pores for cell growth and
differentiation within the microspheres. The characteristics of open
macroporous microspheres are desirable for high cell-seeding and
cell-culture density.
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