Zebrafish: A Predictive Model for Assessing Cancer Drug-Induced Organ Toxicity - Zebrafish: Methods for Assessing Drug Safety and Toxicity - page 137

Figure 11.2 Compound-induced toxicity in zebrafish heart, CNS, liver, and kidney after 24 h treatment.
Control- and compound-treated zebrafish at 8magnification. Heart andCNS (left panels 3dpf): 17-DMAG
causedCNS toxicity (white star) and pericardial edema (black arrow). HDAC inhibitor X caused pericardial
edema (black arrow). Paclitaxel and SMA-838 caused no toxicity in heart or CNS. Liver and kidney (right
panels 5dpf): 17-DMAG caused liver hemorrhage (yellow outline) and trunk edema (red arrow). HDAC
inhibitor X and paclitaxel caused no discernable effects. Seventy percent of SMA-838-treated zebrafish
appeared normal. Scale bar ¼0.5mm. (See the color version of this figure in Color Plates section.)

Table 11.4 Organ Toxicity After 24 h Treatment (Concentration)

Compound Cardiotoxicity CNS toxicity Liver toxicity Kidney toxicity

17-DMAG þ (1500 mM) þ (1500 mM) þ (1500 mM) þ (1500 mM)

HDAC inhibitor X þ (400 mM)

Paclitaxel a

SMA-838 þ (2000 m M)

“þ”: toxicity was observed; “”, no toxicity was observed. The value given in parentheses indicates
concentration at which toxicity was initially observed, which was also the highest concentration tested for
each compound.
a

Tested up to 2 mM, the highest soluble concentration.