Biomedical Engineering Reference
In-Depth Information
FIGURE 7.27 MeOH exposure does not increase 8-oxodG levels in bone
marrow and spleen of Ogg1 (
) mice. Mice were given a
single i.p. dose of 2.0 g/kg bw MeOH (20% [w/v] in sterile saline) or saline
vehicle control, and sacrificed at 6 and 24 hours post-injection. Genomic DNA
was isolated and analyzed for oxidative DNA damage reflected by the
formation of 8-oxodG. Values are means
þ
/
þ
)orOgg1 (
/
4. Symbol
denotes a
difference in Ogg1 (
/
) sample compared to the respective group in Ogg1
(
þ
SE; N
¼
þ
/
þ
) mice (p
<
0.05). Source: Modified from McCallum et al. (2011a,b).
Parthasarathy et al., 2006b) determined by the controversial TBARS
method (see Section 7.4.1.3), we conducted an additional measure of
free radical-initiated macromolecular damage by measuring the HNE-
histidine (HNE-His) protein adducts under conditions minimizing the
influence of adventitious iron in cellular homogenates (see Section
7.4.1.3 for carcinogenic relevance). We detected modest increases in the
levels of the HNE-His adduct only in mouse bone marrow (1.4-fold) and
in rabbit spleen (1.5-fold) with no increases observed in primate
bone marrow or spleen or mouse spleen (McCallum et al., 2011a)
(Figure 7.28). For comparison, acute exposure to 20mg/kg i.p. of the
