Oxidative Stress and Species Differences in the Metabolism, Developmental Toxicity, and Carcinogenic Potential of Methanol and Ethanol - The Toxicology of Methanol - page 234

FIGURE 7.28 HNE-His protein adducts in bone marrow and spleen from
male CD-1 mice, New Zealand white rabbits, or cynomolgus monkeys
following acute exposure to MeOH (2.0 g/kg i.p.). Animals were given a
single i.p. dose of 2.0 g/kg bw MeOH (20% [w/v] in sterile saline) or saline
vehicle (control) and sacrificed at 6 or 24 hours post-injection. Values are
means

3 for rabbits and monkeys. The symbol
denotes a difference from saline control (p

þ SE; N ¼ 4 for mice and N ¼

< 0.05). Source: Modified from

McCallum et al. (2011a).

redox cycling agent doxorubicin increased the levels of HNE-His
protein adducts by 5.3-fold in mouse heart homogenates (Mukhopad-
hyay et al., 2010). In contrast, the absence of MeOH-initiated HNE-
His protein adducts in mouse spleen, and more importantly in either
primate bone marrow or spleen, or in rabbit bone marrow, indicates this
oxidative effect of MeOH is limited and unlikely to occur to a biolo-
gically significant degree in adult humans. In general, these observa-
tions suggest that a limit dose of MeOH (2.0 g/kg i.p.) can produce
modest oxidative stress, but the potential for macromolecular damage is
highly specific with respect to macromolecular target, species, and