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protein profiles. This discriminant model allowed the correct classification of most soybean
varieties (90.7%) being peaks 2 and 5 those showing the highest discriminant capabilities.
Moreover, the discriminant analysis methodology was also useful for the differentiation
among soybean varieties original from different USA states observing the correct
classification of 97.1% of the American soybeans studied. Peak area percentages also allowed
the estimation of the 11S/7S ratios in all 91 soybean cultivars observing that they ranged from
0.46 to 3.45 and detecting the highest ratios in Asian varieties and the lowest in European
ones. Certain correlations among the 14 chromatographic variables (peak area percentages)
and the palmitic and linoleic acids contents were also observed. All these results seem to
demonstrate the chromatographic protein profiles are characteristic of a cultivar and could be
useful for characterization purposes.
A
CKNOWLEDGMENTS
The authors gratefully acknowledge financial support from the Comunidad Autónoma de
Madrid, Spain (project S-0505/AGR/0312) and the Ministerio de Ciencia y Tecnología, Spain
(project AGL2005-05320-C02-01/ALI). Dr. M. C. García also thanks financial support
received from the Comunidad Autónoma de Madrid (project CCG07-UAH/AMB-1831). J. N.
Ovalles is acknowledged for technical assistance. Finally, the authors thank the kind donation
of the 91 soybean varieties by Dr Lucía de la Rosa (CRF-INIA, Madrid, Spain).
R
EFERENCES
[1]
Natarajan, S., Xu, C., Bae, H., Caperna, T. J., Garrett, W. M. Characterization of
storage proteins in wild (Glycine soja) and cultivated (Glycine max) soybean seed
using proteomic analysis.
J. Agric. Food Chem
., 2006, 54, 3114-3120.
[2]
Wang, C.; Wu, X.; Jia, F.; Zhang, J.; Chen, S. Genetic variations of glycinin subunit
genes among cultivated and wild type soybean species.
Progress Nat. Sci
., 2008, 18,
33-41.
[3]
Zarcadas, C. G.; Gagnon, C.; Poysa, V.; Khanizadeh, S.; Cober, E. R.; Chang, V.;
Gleddie, S. Protein quality and identification of the storage protein subunits of tofu and
null soybean genotypes, using amino acid analysis, one- and two-dimensional gel
electrophoresis, and tandem mass spectrometry.
Food Res. Int
., 2007, 40, 111-128.
[4]
Clarke, E. J.; Wiseman, J. Developments in plant breeding for improved nutritional
quality of soya beans I. Protein and amino acid content.
J. Agric. Sci
., 2000, 134, 111-
124.
[5]
Krishnan, H. B.; Bennett, J. O.; Kim, W. S.; Krishnan, A. H.; Mawhinney, T. P.
Nitrogen lowers the sulfur amino acid content of soybean (
Glycine max [L] Merr
.) by
regulating the accumulation of Bowman-Birk protease inhibitor.
J. Agric. Food Chem
.,
2005, 53, 6347-6354.
[6]
Riblett, A. L.; Herald, T. J.; Schmidt, K. A.; Tilley, K. A. Characterization of β-
conglycinin and glycinin soy protein fractions from four selected soybean genotypes.
J.
Agric. Food Chem
., 2001, 49, 4983-4989.
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