Biology Reference
In-Depth Information
Fig. 11.
Log/log plot of concentration vs. the yield of virus production
for procapsid and gp9. (Adapted from Ref. 151, the author and the pub-
lisher, Elsevier.)
12.1. This means that the theoretical value of the sto-
ichiometry of gp12 is 12. This agrees with the stoichiometry of gp12
that has been determined previously.
138-141
The issue of the reusability of components can interfere with and
complicate the results when using the log/log slope assay method for
stoichiometric determination. The principle for the determination of
stoichiometry by this method is based on the concentration of the
components. If a component is reusable, the outcome of quantifica-
tion will not be reliable in comparison to components that are non-
reusable. If the component is reusable, the recycled molecules will
add to the pool of molecules that are able to function in
in vitro
assembly. Therefore, the concentration will appear higher than it
actually is, thus the stoichiometry determined will be lower than the
real number.
T
=
5.67,
Z
=
