Biology Reference
In-Depth Information
Fig. 10.
Dose-response curves with log/log plot showing DNA-gp3,
pRNA, gp16, gp11, and gp12 concentration dependence. The slopes of the
curves for DNA are 1 (45?), suggesting that one DNA is needed for assembly
of one virion. (Adapted from Ref. 151, the author and the publisher, Elsevier.)
yield of the reaction, the stoichiometry
Z
can be determined with
an equation (Eq. (4)),
Z
=−
1.58
+
2.4193
T
−
0.001746
T
2
[
T
∈
(0, 1000),
(4)
or
∠α ∈
(0
°
, 89.9
°
)]
where
T
is the slope of the curve (i.e. the tangent of
a), which was
defined as the angle between the curve and the
x
-axis. This equation
is also examined for its feasibility in stoichiometry determination
(Tables 6 and 7). It was found that the stoichiometry could be deter-
mined by these formulas with high reliability. For example, the angle
of the curve representing gp12 was determined to be 80
∠
°
, so
T
=
tan
80
°=
5.67. From equation
Z
=−
1.58
+
2.4193
T
−
0.001746
T
2
, if
