Biology Reference
In-Depth Information
Fig. 9.
Log/log plot of concentration vs. the yield of virus production for
pRNA monomer, dimer, and trimer. (Adapted from Ref. 151, with permis-
sion from the author and the publisher, Elsevier.)
their stoichiometry. A computer program, such as Cricket Graph, can
be used to find a best-fit curve and to deduce the formula for stoi-
chiometric determination.
Several formulas have been tested for their suitability in the sto-
ichiometric determination of viral assembly components in various
circumstances. These formulas include polynomial (
Z
=
m
+
k
1
T
+
+
...
), logarithmic [
Z
k
2
T
2
=
(
m
+
k
*log(
T
)], exponential (
Z
=
kT
) equations, where
Z
is the stoi-
chiometry,
T
is the slope that is equal to the tangent of the angle
between the curve and the
x
-axis, and
k
and
m
are constants that
are determined empirically. In the experimental data from phi29
(Table 5), the polynomial with two orders is the best fit, as sug-
gested by the
R
2
value of 0.998 (Fig. 12). When the log of dose
response curves or dilution factor is plotted against the log of the
mn
kT
), and linear (
Z
=
m
+
