Biology Reference
In-Depth Information
Table 5. Stoichiometry and Slopes for phi29 Components, such as Procapsid, DNA-gp3, pRNA Trimer, Dimer and
Monomer, as well as gp9, gp11 and gp12, obtained by
In Vitro
Viral Assembly System
Components for
Total
∠
of
Slopes
Slopes
Phi29 Virion
Subunits/
Curve
(tan)
(tan)
Assembly
Function
Virion
Stoichiometry
(Degrees)
Empirical
Theoretical
DNA-gp3
Genome complex
1
1
45
±
4
1
1
Procapsid
Hold DNA
1
1
47
±
3
1.07
1
pRNA trimer
DNA-packaging
6
2
56
±
2
1.48
1.48
pRNA dimer
DNA-packaging
6
3
64
±
5
2.05
2.05
pRNA monomer
DNA-packaging
6
6
72
±
3
3.08
3.14
Gp9
Tail knob protein
10
9
77
±
2
4.33
4.33
Gp11
Lower collar
12
12
79
±
1
5.14
5.67
Gp12
Anti-receptor
24
12
80
±
2
5.67
5.67
(Adapted from Ref. 151, with permission from the author and the publisher, Elsevier.)
