Biomedical Engineering Reference
In-Depth Information
Table 8
Tropane alkaloid analysis by LC-MS and LC-MS/MS applied to intoxication and cases of drug abuse
Column (mm × mm)/ fl ow [ml/min]/
T
(°C)
Ionization/mass
analyser/scan mode
Analyte
Mobile phase
a
(run mode)
Lin range (ng/ml)
b
Reference
Analysis of plasma, serum or whole blood
Atr
C18 Hypurity Aquastar Javelin Express
(50 × 2.1)/0.2/n.s.
0.13 M NH
4
OAc +30 mM TFA/H
2
O/ACN
(gradient)
5-100
+ESI/QqQ/MRM
[
53
]
Atr, Scp
Superspher60 RP Select B
(125 × 2)/04-0.6/25
ACN/50 mM NH
4
FA, pH 3.5 (gradient)
APCI
Atr: 5-100
Scp: 5-100
ESI
Atr: 0.1-100
Scp: 0.1-100
+APCI/SQ/SIM
+ESI/QqQ/MRM
[
11
]
Ipra
Atlantis dC18 (100 × 2.1)/0.2/30
Solv A: 15 mM HFBA, 20 mM NH
4
FA, pH 3.3
Solv B: MeOH (gradient)
19.5-200
+ESI/IT/MRM
[
54
]
Analysis of urine
Atr
MB Nucleodur C18 (150 × 1)/0.05/n.s.
Solv A: 2 mM NH
4
FA, pH 3.0
Solv B: ACN/solv A 90:10 (gradient)
n.s.
+ESI/QqQ/MRM
[
12
]
Atr, Scp
Hypersil GOLD (100 × 2.1)/0.2/n.s.
Solv A: ACN/10 mM FA 1:99
Solv B: ACN/10 mM FA 60:40 (gradient)
Atr: 5-5,000
Scp: 10-5,000
+ESI/QqQ/MRM
[
13,
55
]
Atr, Scp
Luna C18(2) (150 × 2)/0.25/40
Solv. A: 10 mM NH
4
FA in water
Solv. B: 10 mM NH
4
FA in MeOH (gradient)
n.s.
+APCI/IT/MRM
[
14
]
Ipra, BuS Suplecosil LC-8-DB (100 × 2.1)/0.2/30
Solv A: 10 mM NH
4
FA, pH 3.0
Solv B: ACN (gradient)
n.s.
+ESI/IT/MRM
[
78
]
Analysis of hair
Scp
ACQUITY C18 (50 × 2.1,
1.7 m m)/0.3/n.s.
Solv. A: ACN
Solv. B: 2 mM NH
4
FA, pH 3.0 (gradient)
0.2-20 pg/mg
+ESI/QqQ/MRM
[
56
]
