Biomedical Engineering Reference
In-Depth Information
Analyte
(°C)
Mobile phase
a
(run mode)
Lin range (ng/ml)
b
Ionization/mass
analyser/scan mode Reference
Atr,
R
-/
S
-Hyo
i
Atlantis T3 C18 (150 × 4.6)/1.0/30
Solv A: 0.1 % v/v FA
Solv B: ACN/0.1 % FA 80:20 (gradient)
0.1-50
+ESI/QqQ/MRM
[
49
]
For abbreviation of analyte names see Sect. “Abbreviations”.
ACN
acetonitrile,
EBQ
1
Q
2
double focusing sector field mass spectrometer,
ESI
electrospray ion-
ization,
FA
formic acid,
FA B
fast atom bombardment,
hep-SA
heptanesulfonic acid,
iso
isocratic,
IT
ion trap,
lin range linear range
,
MeOH
methanol,
MRM
multiple reaction monitoring,
MS
full scan mass spectrometry,
MS/MS
tandem mass spectrometry,
n.s. not specified
,
OAc
acetate,
QqQ
triple quadrupole mass
spectrometer,
SIM
selected ion monitoring,
Solv
HPLC solvent,
SQ
single quadrupole mass spectrometer,
T
temperature,
TSP
thermospray
a
Ratios given as v/v
b
Lower limit of linear range corresponds to lower limit of quantification
c
In vivo: rat urine, plasma, faeces, in vitro: rat liver homogenates
d
In vivo: dog and monkey urine and plasma
e
In vivo: rat urine and bile
f
In vitro: liver microsomes from rat, mouse, hamster, and guinea pig
g
In vivo: rat urine
h
Human liver microsomes
i
In vitro: human and rabbit serum
j
The low flow published (6 ml/min) seems to be erroneous
