Biomedical Engineering Reference
In-Depth Information
Table 7
Tropane alkaloid analysis by LC-MS and LC-MS/MS for biotransformation studies in vivo and in vitro
Column (mm × mm)/ fl ow (ml/min)/
T
(°C)
Ionization/mass
analyser/scan mode Reference
Analyte
Mobile phase
a
(run mode)
Lin range (ng/ml)
b
Ada
c
AICHROM ReliAsil C18
(150 × 2)/0.2/40
MeOH/0.01 % Et
3
N, pH 3.5, 60:40 (iso)
n.s.
±ESI/IT/MS/MS
[
6
]
Adi
c
AICHROM ReliAsil C18
(150 × 2)/0.2/40
MeOH/0.01 % Et
3
N, pH 3.5, 60:40 (iso)
n.s.
±ESI/IT/MS/MS
[
5
]
Atr
c
Zorbax extend C18 (100 × 3)/0.2/40
MeOH/2 mM NH
4
OAc, pH 3.5 70:30 (iso)
n.s.
+ESI/IT/MS/MS
[
51,
52
]
Beme
d
Spherisorb CN (250 × 4.6) or Spherex
C-18 (250 × 4.6)/1.0/n.s.
Solv A: 0.05 M NH
4
OAc, pH 4.4
Solv B: 0.05 M NH
4
OAc/ACN 55:45
Solv C: 0.05 M NH
4
OAc/ACN 40:60 (gradient)
n.s.
+TSP/SQ/n.s.
[
37
]
Benz
e
CSC spherisorb cyano
(100 × 25)/0.006
j
/n.s.
H
2
O/ACN 80:20, 0.1 % HOAc (iso)
n.s.
+ESI/SQ/MS
[
59
]
Cim
f
Spherisorb ODS-2 (250 × 4.6)/1.0/n.s. 20 or 35 mM hep-SA, 100 mM Et
3
N in MeOH/
ACN/H
2
O 20:20:55 or 5:10:55 , pH 3.7 (iso)
30-150
+FAB (Xe)/EBQ
1
Q
2
/
MS/MS
[
23,
62
]
Scp
g
Zorbax extend C18 (100 × 3)/0.2/40
MeOH/2 mM NH
4
OAc, pH 3.5 70:30 (iso)
n.s.
+ESI/IT/MS/MS
[
7,
87
]
Trop
h
Nucleosil C4 (250 × 4.6)/0.5/35
Solv A: ACN/20 mM NH
4
OAc 10:90, pH 6.0
Solv B: ACN/20 mM NH
4
OAc 60:40, pH 6.0
(gradient)
70-1,425
+ESI/SQ/SIM
[
82
]
Atr, BuS, Coc,
Hatr, Ipra,
Lit, Scp
i
Atlantis T3 C18 (150 × 4.6)/1.0/30
Solv A: 0.1 % v/v FA
Solv B: ACN/0.1 % FA 80:20 v/v (gradient)
Atr, Coc, Ipra, Lit,
Scp: 0.05-50
Hatr: 0.1-50
BuS: 0.8-50
+ESI/QqQ/MRM
[
50
]
R
-/
S
-Hyo
i
AGP (2x 150 × 2)/0.3/40
Solv A: 10 mM NH
4
FA, pH 8.0
Solv B: 10 mM NH
4
FA in 25 % ACN, pH 8.0;
85:15 (iso)
1-500
+ESI/QqQ/MRM
[
49
]