Biomedical Engineering Reference
In-Depth Information
Analyte
(°C)
Mobile phase
a
(run mode)
Lin range (ng/ml)
b
analyser/scan mode
Reference
Atr, Scp
XTerra MS C18 (100 × 2.1)/0.2/n.s.
ACN/formate buffer (gradient)
Atr: 5-100 pg/mg
Scp: 5-100 pg/mg
+ESI/QqQ/MRM
[
57
]
Analysis of hum viscera
Atr, Scp
Xterra Phenyl (150 × 2.1)/0.2/n.s.
ACN/10 mM NH
4
OAc, pH 10.5 (gradient)
Atr: 100-10,000
Scp: 100-10,000
+ESI/SQ/SIM
[
15
]
For abbreviation of analyte names see Sect. “Abbreviations”.
ACN
acetonitrile,
APCI
atmospheric pressure chemical ionization,
ESI
electrospray ionization,
FA
formic acid,
HFBA
hepta fl uorobutyric acid,
IT
ion trap,
lin range
linear range,
MeOH
methanol,
MRM
multiple reaction monitoring,
n.s.
not speci fi ed,
OAc
acetate,
QqQ
triple quadrupole mass spectrometer,
SIM
selected ion monitoring,
Solv
HPLC solvent,
SQ
single quadrupole mass spectrometer,
T
temperature,
TFA
tri fl uoroacetic acid
a
Ratios given as v/v
b
Lower limit of linear range corresponds to lower limit of quantification
