Therapeutic Drug Monitoring of Targeted Anticancer Therapy. Tyrosine Kinase Inhibitors and Selective Estrogen Receptor Modulators: A Clinical Pharmacology Laboratory Perspective - LC-MS in Drug Bioanalysis - page 229

Matrix (volume) Analytes e Internal standard (ng/ml) Sample preparation size, dimensions) detection mode Reference

Plasma
(100 m l)

Endoxifen Anastrozole - PP (ACN) Kromasil 100 C8

(5 m m,
150 × 4.6 mm)

ESI-TSQ [ 243, 244 ]

Plasma b
(100 m l)

Tam
N -D-Tam
4-OH-Tam
4 ¢ -OH-Tam c
Endoxifen d
4 ¢ -OH- N -D-Tam c

Tam-d5
N -D-Tam-d5
4-OH-Tam-d5

1
2
0.4
0.4
1
1

PP (ACN)
Evaporation
Reconstitution
(MeOH/20 mM
ammonium
formate buffer,
pH 2.9 (1:1 v/v))
Centrifugation

Acquity UPLC BEH
C18 (1.7 m m,
30 × 2.1 mm)

ESI-TSQ [ 145 ]

Endoxifen-d5 d

Plasma b
(100 m l)

Tam
Tam-NO
N -D-Tam
4-OH-Tam d
4 ¢ -OH-Tam
Endoxifen d
4 ¢ -OH- N -D-Tam

Tam- 13 C 2 , 15 N 1.1

-
0.5
0.2
0.2
0.2
0.2

PP (MeOH)
Filtration
Dilution (H 2 O)

Zobrax SB-C18
(1.8 m m,
50 × 2.1 mm)

ESI-TSQ [ 245 ]

N -D-Tam-d5
4-OH-Tam-d5

Endoxifen-d5 d

Serum
(200 m l)

Tam
N -D-Tam
4-OH-Tam
Endoxifen

Tam-d5
N -D-Tam-d5

-
-
-
-

Polymer-based
mixed-mode SPE
(Oasis MCX 1 ml
cartridges)

XTerra MS C18
(3.5 m m,
100 × 2.1 mm)

ESI-LTQ [ 173 ]

Endoxifen-d5

(continued)