Biomedical Engineering Reference
In-Depth Information
Table 3
(continued)
LOQ
(ng/ml)
Column (particle
size, dimensions)
Ionization and
detection mode
Matrix (volume)
Analytes
e
Internal standard
Sample preparation
Reference
Serum
(1 ml)
Tam
N -D-Tam
4-OH-Tam
-
-
-
5
5
0.5
LLE (
n
-hexane/
isoamyl alcohol
(98:2))
Evaporation
Reconstitution
(MeOH)
Beckman C8 (5 m m,
50 × 4.6 mm)
ESI-LTQ
[
241
]
Serum
Tam
4-OH-Tam
Propranolol
10
1
LLE (hexane/
isopropanol
(95:5 v/v))
HiQ-Sil C18 (5 m m,
150 × 2.1 mm)
ESI-TSQ
[
194
]
Serum
b
(50 m l)
Tam
Tam-NO
c
N -D-Tam
4-OH-Tam
Endoxifen
d
Soy iso fl avone
(genistein,
daidzein,
glycitein)
Tam-d5
6.76
6.19
6.72
1.13
2.69
PP (ACN)
Dilution (3.5 mM
ammonium
formate buffer,
pH 3.5)
Synergi Hydro-RP
(4 m m,
150 × 2 mm)
ESI-TSQ
[
242
]
N -D-Tam-d5
4-OH-Tam-d5
Endoxifen-d5
d
(genistein-d4,
daidzein-d6)
Plasma
(100 m l)
Tam
N -D-Tam
4-OH-Tam
Endoxifen
Imipramine
20
20
1
3.75
PP (ACN)
SPE (BOND
ELUTE-C18
cartridges,
100 mg/1 ml)
XBridge C18
(3.5 m m,
150 × 3 mm)
ESI-TOF
[
195
]
Plasma
b
(1 ml)
Tam
AIs (Anastrozole,
letrozole)
Bunitrolol
25
PP (2 % aqueous
phosphoric acid)
Polymer-based
mixed-mode SPE
(Strata X-C,
200 mg/3 ml)
Eurosphere Si-C18
(5 m m,
200 × 0.5 mm)
ESI-LTQ
[
218
]
