Biomedical Engineering Reference
In-Depth Information
Table 3
(continued)
LOQ
(ng/ml)
Column (particle
size, dimensions)
Ionization and
detection mode
Matrix (volume)
Analytes
e
Internal standard
Sample preparation
Reference
Plasma
(50 m l)
Tam
Tam-NO
N -D-Tam
N -D-D-Tam
4-OH-Tam
d
3-OH-Tam
4 ¢ -OH-Tam
d
a -OH-Tam
Endoxifen
d
3-OH- N -D-Tam
c
4 ¢ -OH- N -D-Tam
d
a - OH- N -D-Tam
c
Tam- N
+
-Gluc
Tam-3- O -Gluc
c
Tam-4-
O
-Gluc
d
N -D-Tam-3- O -
Gluc
c
N -D-Tam-4- O -
Gluc
d
Tam-d3
0.5
0.2
1
0.2
0.1
0.1
0.1
0.02
0.1
-
0.05
-
0.05
-
0.1
-
PP (ACN + 1 % acetic
acid)
Dilution (H
2
O + 1 %
acetic acid)
Zobrax Eclipse plus
C18 (1.8 m m,
100 × 2 mm)
ESI-TSQ
[
196
]
N -D-Tam-d5
4-OH-Tam-d5
d
Endoxifen-d5
d
Tam-4-O-Gluc-d5
d
0.05
Serum
b
(50 m l)
Tam
N -D-Tam
4-OH-Tam
4 ¢ -OH-Tam
Endoxifen
d
4 ¢ -OH- N -D-Tam
Tam-d5
N -D-Tam-d5
4-OH-Tam-d5
5
5
0.4
0.2
1
1
PP (ACN)
Evaporation
Reconstitution
(ACN/4 mM
ammonium
formate buffer,
pH 3.5, (3:7 v/v))
Kinetex C18
(2.6 m m,
150 × 2.1 mm)
APCI-TSQ
[
246
]
Endoxifen-d5
d
Plasma
(250 m l)
Tam
N -D-Tam
4-OH-Tam
Endoxifen
Diphenhydramine
-
-
-
-
LLE (ethyl acetate
under pH 11.3)
Evaporation
Reconstitution
(mobile phase)
Luna C18 (3 m m,
100 × 2 mm)
ESI-TSQ
[
226
]
