Biomedical Engineering Reference
In-Depth Information
Author, year [Ref]
Compounds
IS
Sample
Extraction procedure
Stationary phase
Mobile phase
mode)
Method validation
Castaing et al.,
2007 [
109
]
Fluoxetine, paroxetine,
sertraline, fl uvoxamine,
citalopram, milnacipram,
venlafaxine, mirtazapine
(and some metabolites)
Methylrisperidone
Whole blood
(0.5 mL)
LLE: hexane/
isoamylic alcohol
(99:1); organic
layer back-
extracted with
HCl 0.05N
Xterra RP18
(100 × 2.1 mm)
4 mM
ammonium
formate pH
3.2/ACN
ESI+/QqQ
(MRM)
-
Selectivity
-
LLOQ = 5-20 ng/mL
-
Linearity: 5/20 to
500/2,000 ng/mL;
r
2
> 0.99
-
Intra-/inter-day
imprecision: %CV < 15%
-
Inter-day inaccuracy:
MRE ± 15%
-
Relative
recovery = 10-85%
-
Matrix effect <20%
-
Dilution integrity (1/10):
MRE ± 15%
-
Application to real
specimens
de Castro et al.,
2007 [
72
]
Amitriptyline, nortriptyline,
imipramine, desipramine,
trazodone, fl uoxetine,
paroxetine, fl uvoxamine,
sertraline
Imipramine-d3,
desipramine-d3,
clomipramine-d3,
fl uoxetine-d6,
paroxetine-d6
Plasma
(0.05 mL)
Online SPE: Oasis
MCX Prospekt
cartridge
Gemini C18
(150 × 2 mm, 5 m m)
Gemini C18 guard
column (4 × 2 mm,
5 m m)
10 mM
ammonium
hydrogencar-
bonate pH
10/ACN
ESI+/QqQ
(MRM)
-
Selectivity
-
LOD = 0.5-1 ng/mL
-
LLOQ = 10 ng/mL
-
Linearity: 10-1,000 ng/
mL;
r
2
> 0.99
-
Intra-/inter-day
imprecision: %CV < 15%
-
Inaccuracy: MRE ± 15%
-
Relative recovery >99%
-
Matrix effect <20%
-
Autosampler (6 ± 2 °C/24
and 48 h) and freeze/thaw
stability
-
Application to real
specimens
(continued)
