Biomedical Engineering Reference
In-Depth Information
Table 1 (continu ed)
IM/analyzer
(detection
mode)
Author, year [Ref]
Compounds
IS
Sample
Extraction procedure
Stationary phase
Mobile phase
Method validation
Santos-Neto et al.,
2008 [ 77 ]
Fluoxetine, imipramine,
desipramine, amitriptyline,
nortriptyline
Fluoxetine-d5
Plasma/Urine
(0.2 mL)
LiChrospher ADS-C18
capillary RAM
extraction
precolumn (60 Å,
25 m m)
YMC ODS-AQ capillary
column (120 Å, 5 m m)
20 mM ammonium
formate pH 5.4/
ACN
ESI+/QqQ
(MRM)
In plasma:
-
LLOQ = 1 ng/mL
-
Linearity: 1-250 ng/mL;
r 2 > 0.99
-
Intra-/inter-day
imprecision: %CV < 20%
-
Inter-day inaccuracy:
MRE ± 20%
-
Relative recovery >80%
-
Matrix effect (post-col-
umn infusion experiment)
-
Carry over: 0.3% signal
from the previous
injection
Breaud et al.,
2010 [ 78 ]
Amitriptyline, nortrityline,
imipramine, desipramine
Amitrityline-d3,
nortriptyline-d3,
imipramine-d3,
desipramine-d3
Plasma (10 m L)
Turbulent- fl ow,
Cyclone-P column
(0.5 × 50 mm)
Hypersil Gold C18
(50 × 3 mm, 5 m m)
0.1% formic acid/
ACN (0.1%
formic acid)
ESI+/QqQ
(MRM)
-
Selectivity
-
LOD = 2-3 ng/mL
-
LLOQ = 9-18 ng/mL
-
Linearity: 69/74 to
1131/1155 ng/mL;
r 2 > 0.99
-
Intra-/inter-day
imprecision: %CV < 20%
-
Intra-day inaccuracy:
MRE ± 15%
-
External pro fi ciency test:
±2 SD of target
concentration
-
Matrix effect <20%
-
Carry over
-
Application to real
specimens
 
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