Biomedical Engineering Reference
In-Depth Information
Table 1
(continu
ed)
IM/analyzer
(detection
mode)
Author, year [Ref]
Compounds
IS
Sample
Extraction procedure
Stationary phase
Mobile phase
Method validation
Alves et al.,
2007 [
80
]
Amitriptyline, nortrityline,
imipramine, desipramine
Clomipramine
Plasma (1 mL)
Online SPME
(PDMS/DVB,
60 m m)
Zorbax XDB RP-18
(150 × 2.1 mm, 5 m m)
RP8 guard column
ammonium
acetate/ACN
ESI+/Q (SIM)
-
Selectivity
-
LOD = 0.1 ng/mL
-
LLOQ = 50 ng/mL
-
Linearity: 50-500 ng/mL;
r
2
> 0.985
-
Intra-/inter-day
imprecision: %CV < 15%
-
Absolute
recovery = 1.2-7.7%
Titier et al.,
2007 [
60
]
Amitriptyline, clomipramine,
trimipramine, imipramine,
doxepin, mianserin,
maprotiline, dosulepine,
amoxapine, toloxatone,
moclobemide, viloxazine
(and some metabo-
lites) + two other
pharmaceuticals
Methylrisperidone
Whole blood
(1 mL)
LLE: hexane/
isoamylic alcohol
(99:1); organic
layer back-
extracted with
HCl 0.02N
Xterra RP18
(100 × 2.1 mm)
4 mM
ammonium
formate pH
3.2/ACN
ESI+/QqQ
(MRM)
-
Selectivity
-
LLOQ = 2 ng/mL
-
Linearity: 5-100 ng/mL;
r
2
> 0.99
-
Intra-/inter-day
imprecision: %CV < 22%
-
Inaccuracy: MRE ± 15%
-
Relative
recovery = 13-82%
-
Matrix effect <16%
-
Dilution integrity (1/10
and 1/50): %CV <15%,
MRE ± 15%
-
Application to real
specimens
