Biomedical Engineering Reference
In-Depth Information
TABLe 4.1 (continued)
Applications.of.Biomolecular.Motors.in.Nanotechnology
Motor
Guidance
Experiment
Future Function/Application
Viability
Reference
NKHK560.cys:.
H. sapien
.
kinesin.stalk.
(residues.
430-560).with.
head/neck.
domains.of.
N.
crassa
.kinesin.
(amino.acids.
1-433);.cysteine.
C-terminal.end.
(reactive)
Combination.of.low.
and.glide.for.two.
functional.steps.on.
stand-alone.
microluidic.device
Captured.streptavidin.
molecules.lowing.in.an.
analyte.stream;.removed.by.
functional.MT.shuttles.
seeking.a.horseshoe-shaped.
collector;.MTs.carrying.
analyte.luoresce.at.sensor
Guided.mass-transport.of.
analyte.carrying.molecular.
shuttles.to.particular.
locations.on.NEMS,.MEMS,.
lab-on-a-chip.devices;.
prevention.of.MT.diffusion.
away.from.device.surfaces;.
self-sorting
Proven.experimentally.as.a.
successful.device.for.
concentrating.analytes.to.a.
particular.location.for.sensing;.
incorporation.into.functional.
application.has.not.yet.
occurred
Kim.et.al..
(2009)
D. melanogaster
.
WT.Kin-1.with.
C-term.His.Tag.
(full.length)
Glide;.docked.kinesin.
motors;.motion.occurs.
within.micro-sized.
“smart.dust”.detection.
arena
Relies.on.a.capture-
transport-tag-transport-
detect.series.of.events.using.
antibodies.and.
functionalized.MTs;.similar.
to.double-antibody.
sandwich.assay;.caged.ATP.
for.velocity.control;.
streptavidin-dependent.
detection.coupled.with.
photoexcitation/emission
Drug.delivery.and.sensing.
mechanism;.detection.of.
toxins,.hormones,.etc.;.
integration.into.biomedical.
and.defense.devices;.
lab-on-a-chip.sample.
preparation.and.detection;.
therapeutic.products
Primary.device.has.been.
constructed.and.proven.to.
display.the.capture-transport-
tag-transport-detect.function;.
photo.excitation.and.emission.
shown.to.allow.detection.in.
the.detection.zone.of.the.
arena;.not.yet.reined.for.
applicability.into.
manufacturable.devices.for.
drug.delivery.or.defense
Fischer.et.al..
(2009)
Conventional.
kinesin
Glide;.docked.kinesin.
motors.on.3D.
relective.surfaces;.
motor.density-
dependent.dynamics.
of.rhodamine-labeled.
MTs
Inverted.motility.basis;.MT.
tip.length.(end.of.MT.
shuttle.to.irst.bound.motor).
relects.ability.to.traverse.
obstacles;.positions.of.large.
numbers.of.MTs.traced.over.
a.surface;.images.overlaid.to.
produced.3D.surface.
topography.of.nanometer.
resolution;.Z-component.
inferred.by.MT.brightness
Nano-imaging;.high.
resolution.surface.analysis;.
self-propelled.probing.
devices
Experimentally.proven;.
applicability.limited.by.
particular.problem.statement;.
likely.to.be.trumped.by.other.
methods.of.imaging
Kerssemaker.
et.al..(2009)
