Minimum Reactants Required for Synthesis of Skin and Peripheral Nerves; Extension to Tissues of Other Organs - Tissue and Organ Regeneration in Adults - page 188

Table 7.2 Reaction diagrams for synthesis of a peripheral nerve

No. Reactants Reaction conditions and structure of

product

References

Response of culture medium or defect in the absence of reactants (negative control for all reaction diagrams)
N01

Cell-free medium → no tissues Negative control for reactions in culture

(in vitro); no tissues synthesized

N02 Negative control for reactions in the

adult transected nerve defect (in vivo);
neuroma synthesized

Weiss 1944; Denney-Brown 1946;
Chamberlain et al. 2000a; other
authors; see Chap. 2

in vivo →

neuroma

Transected nerve

A. Myelin sheath (MAX, myelinated axons)
N1 AX + SC + COFL → MAX Schwann cells cultured with neurons

on collagen surface; myelin sheath
synthesized

Bunge and Bunge 1978

N2 AX → No MAX No myelin sheath synthesized around

axons in regions free of Schwann cells

Bunge and Bunge 1978

N3 AX + SC + RBM → MAX Schwann cells cultured with neurons

and reconstituted BM; myelin sheath
synthesized

Carey et al. 1986

N4 AX + SC → MAX Schwann cells cultured with neurons;

myelin sheath synthesized

Carey and Todd 1987; Eldridge et al.
1987, 1989; Clark and Bunge 1989;
Podratz et al. 1998

N5 AX + SC + LA → → MAX Schwann cells cultured with neurons and

laminin; myelin sheath synthesized

Eldridge et al. 1989

B. Basement membrane encasing Schwann cells (BM)
N6 AX → No BM No BM synthesized in absence of

Schwann cells

Bunge et al. 1980

N7 AX + SC → MAX · BM (+ COF) Schwann cells cultured with neurons; BM

synthesized; synthesis of collagen fibrils
occasionally reported

Bunge et al. 1980, 1982; Cornbrooks
et al. 1983; Carey and Todd 1987;
Eldridge et al. 1987, 1989; Clark and
Bunge 1989