Minimum Reactants Required for Synthesis of Skin and Peripheral Nerves; Extension to Tissues of Other Organs - Tissue and Organ Regeneration in Adults

Biomedical Engineering Reference

In-Depth Information

Table 7.1 (continued)

No.

Reactants

Reaction conditions and structure of

product

References

S31

FB and KC cultured on modified

(laminated) DRT, then grafted; simul-

taneous synthesis of epidermis, BM,

rete ridges with dermal papillae and

dermis, including elastic fibers

Cooper and Hansbrough 1991;

Cooper et al. 1993

in vivo →

KC + FB + L-DRT

E · BM · RR · D

S32

KC cultured on collagenous bilayer

to synthesize epidermis, then grafted;

synthesis of BM and dermis; dermal

elastic fibers not reported; no rete

ridge formation

Bosca et al. 1988; Tinois et al. 1991

in vivo →

E · BM · D

KC + CBL

S33

KC cultured on polyglactin mesh

with FB, then grafted; laminin

stained continuously; type IV col-

lagen synthesis not reported; dermis

with capillaries synthesized; dermal

elastic fibers not reported; no rete

ridge formation

Cooper et al. 1991; Hansbrough et al.

1993

in vivo →

KC + FB + PGL

E · BM(?) · D

E. Skin (E · BM · RR · D · App; skin appendages included)

S34

in vivo →

E . BM ·

DRT seeded with dermal papilla cells

(DPC)

Sriwiriyanont et al. 2011, 2012

DPC + DRT

RR · D · App

? next to symbol for tissue product indicates absence of confirmatory evidence for synthesis of physiological tissue, No indicates product not formed. Reac-

tion conditions : “In vivo” over the reaction arrow indicates reaction conducted in a dermis-free defect; its absence above arrow indicates in vitro study. Bold

letters indicate an irreducible reaction diagram

Reactants: KC keratinocytes, FB fibroblasts, DPC dermal papilla cells, COFL cast type I collagen film, COG type I collagen gel produced by fibroblast

contraction, CBL a bilayer consisting of a type IV collagen layer and a type I+III collagen layer, DRT dermis regeneration template, a porous graft copolymer

of type I collagen and chondroitin 6-sulfate (only collagen used in experiment studies after 2000), L-DRT dermis regeneration template laminated on one side