Minimum Reactants Required for Synthesis of Skin and Peripheral Nerves; Extension to Tissues of Other Organs - Tissue and Organ Regeneration in Adults - page 184

Table 7.1 (continued)

No. Reactants Reaction conditions and structure of

product

References

KC + CBL → E · BM

S8 KC cultured on collagenous bilayer;

hemidesmosomes, lamina lucida,
lamina densa, and occasionally
anchoring fibrils, synthesized

Bosca et al. 1988; Tinois et al. 1991

S9 KC formed epidermis in vitro

and was grafted; lamina lucida,
lamina densa, and anchoring fibrils
synthesized

Woodley et al. 1988; Aihara 1989;
Carver et al. 1993b; Cooper et al.
1993: Orgill et al. 1998

in vivo →

E · BM

KC

KC + FB + COG → E · BM

S10 KC added to precultured collagen gel

and fibroblasts; hemidesmosomes,
lamina lucida, lamina densa, anchor-
ing fibrils synthesized

Chamson et al. 1989; Harriger and
Hull 1992; Okamoto and Kitano
1993; Marinkovich et al. 1993;
Tsunenaga et al. 1994; Smola et al.
1998; Stark et al. 1999

S11 KC added to precultured fibroblasts

in modified (laminated) DRT, then
grafted; lamina lucida, lamina densa,
and anchoring fibrils synthesized

Cooper and Hansbrough 1991;
Cooper et al. 1993; Boyce et al. 1993

in vivo →

E · BM

KC + FB + L-DRT

S12

KC + FB + NY → E · BM

KC added to precultured fibroblasts
in nylon mesh; anchoring filaments,
lamina densa, anchoring fibrils, nido-
gen synthesized

Contard et al. 1993; Fleischmajer
et al. 1995

S13

KC → E · BM

KC cultured in defined medium;
hemidesmosomes, anchoring fila-
ments, lamina lucida, lamina densa
synthesized; collagen VII synthesized
but not secreted

Rosdy et al. 1993

S14 KC added to precultured fibroblasts

in nylon mesh, then grafted; continu-

Hansbrough et al. 1993 in vivo →

E · BM(?)

KC + FB + PGL